recombinant human connective tissue growth factor  (BioVendor Instruments)

Journal: Cell reports

Article Title: Directed differentiation of human iPSCs into mesenchymal lineages by optogenetic control of TGF-β signaling

doi: 10.1016/j.celrep.2023.112509

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Basal tenogenic medium was supplemented with additional factors: 50 μg/mL ascorbic acid 2-phosphate (Sigma), 100 ng/mL human recombinant growth/differentiation factor-7 (GDF-7; Peprotech), and after 3 days, 100 ng/mL human recombinant connective tissue growth factor (CTGF; Peprotech).

Techniques: Affinity Purification, Virus, Recombinant, Protease Inhibitor, SYBR Green Assay, Solvent, Plasmid Preparation, Control, Functional Assay, Bicinchoninic Acid Protein Assay, Reverse Transcription, Quantitative Proteomics, Software

Journal: Frontiers in Pharmacology

Article Title: Kinsenoside Protects Against Radiation-Induced Liver Fibrosis via Downregulating Connective Tissue Growth Factor Through TGF- β 1 Signaling

doi: 10.3389/fphar.2022.808576

Figure Lengend Snippet: The key fibrosis-related gene and signaling pathway that are inhibited by KD (A) The results of high-throughput sequencing of the transcriptome showed that the expression of CTGF gene increased after irradiation (B) The results of high-throughput sequencing of the transcriptome showed that the expression of CTGF gene decreased after the administration of KD (C) The results of Real-Time PCR were consistent with the results of the sequencing. The expression of CTGF mRNA increased in the IR group, but decreased in the IR + KD group (D) The results of western blotting were consistent with the results of sequencing. The expression of CTGF protein increased in the IR group, but decreased in the IR + KD group (E) In the transcriptome KEGG gene pathway enrichment map, the TGF-β pathway was closely related to CTGF and RILF (F) The results of tissue TGF-β1 immunohistochemistry indicated that in the IR + KD group the expression of TGF-β1 in the liver tissue decreased compared with the IR group. For all results in this figure, original magnification, ×40 and ×100. Mean ± SEM. * p < 0.05, ** p < 0.01.

Article Snippet: The antibodies included: α -SMA (1:1,000; Servicebio Technology, Wuhan, China), fibronectin (FN), collagen I and connective tissue growth factor (CTGF) (1:1,000; BOSTER Biological Technology, Wuhan, China), TGF- β 1 and Smad2/3 and P-Smad2/3 (1:1,000; Cell Signaling Technology Inc., Danvers, MA), GAPDH and β -actin (1:1,000; Aspen Biological, Wuhan, China).

Techniques: Next-Generation Sequencing, Expressing, Irradiation, Real-time Polymerase Chain Reaction, Sequencing, Western Blot, Immunohistochemistry

Journal: Frontiers in Pharmacology

Article Title: Kinsenoside Protects Against Radiation-Induced Liver Fibrosis via Downregulating Connective Tissue Growth Factor Through TGF- β 1 Signaling

doi: 10.3389/fphar.2022.808576

Figure Lengend Snippet: KD ameliorates the RILF by inhibiting TGF-β1/Smad/CTGF pathway (A) Exogenous TGF-β1 led to the increased expression of CTGF protein (B) Exogenous TGF-β1 induced an increase in the expression of collagen and a -SMA proteins (C) The TGF-β1 activity in the conditioned media significantly increased after 6Gy irradiation compared with the control group, while the TGF-β1 activity in the conditioned media in the IR + KD group decreased compared with the IR group (D-G) The expressions of TGF-β1 and downstream Smad2/3 as well as phosphorylated Smad2/3 reduced in the IR + KD group compared with the IR group. Mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The antibodies included: α -SMA (1:1,000; Servicebio Technology, Wuhan, China), fibronectin (FN), collagen I and connective tissue growth factor (CTGF) (1:1,000; BOSTER Biological Technology, Wuhan, China), TGF- β 1 and Smad2/3 and P-Smad2/3 (1:1,000; Cell Signaling Technology Inc., Danvers, MA), GAPDH and β -actin (1:1,000; Aspen Biological, Wuhan, China).

Techniques: Expressing, Activity Assay, Irradiation, Control

Journal: Frontiers in Pharmacology

Article Title: Kinsenoside Protects Against Radiation-Induced Liver Fibrosis via Downregulating Connective Tissue Growth Factor Through TGF- β 1 Signaling

doi: 10.3389/fphar.2022.808576

Figure Lengend Snippet: The schematic diagram. KD ameliorated the RILF through the regulation of the key molecule CTGF and TGF-β1/Smad/CTGF pathway in the HSCs.

Article Snippet: The antibodies included: α -SMA (1:1,000; Servicebio Technology, Wuhan, China), fibronectin (FN), collagen I and connective tissue growth factor (CTGF) (1:1,000; BOSTER Biological Technology, Wuhan, China), TGF- β 1 and Smad2/3 and P-Smad2/3 (1:1,000; Cell Signaling Technology Inc., Danvers, MA), GAPDH and β -actin (1:1,000; Aspen Biological, Wuhan, China).

Techniques: